Biomaterial Database

Asymmetric hybrids between hemoglobin Alberta (alpha 2 beta 2 101 Glu replaced by Gly) and hemoglobins A, A2 or F1 in different liganded states. 1980

C A Harasym, and R A Stinson

Isoelectric focusing in cylindrical polyacrylamide gels has been used to demonstrate the formation of asymmetric hybrids between an abnormal hemoglobin, Hb Alberta (alpha 2 beta 2 101 Glu replaced by Gly), and Hb A (alpha 2 beta 2), Hb A2 (alpha 2 delta 2) or Hb F1 (alpha 2 gamma 2 Acetyl). There was no discernible difference in the stabilities of the three hybrids (alpha 2 beta A beta Alberta, alpha 2 delta beta Alberta and alpha 2 gamma Acetyl beta Alberta) in the oxy, carboxy or cyanmet hemoglobin forms. Two mixed liganded hybrids, (alpha beta A)oxy (alpha beta Alberta)cyanmet and (alpha beta A)cyanmet (alpha beta Alberta)oxy were also demonstrated and found to have stabilities similar to that of the Hb Alberta hybrids.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D008024	Ligands	A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)	Ligand
D004355	Drug Stability	The chemical and physical integrity of a pharmaceutical product.	Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005319	Fetal Hemoglobin	The major component of hemoglobin in the fetus. This HEMOGLOBIN has two alpha and two gamma polypeptide subunits in comparison to normal adult hemoglobin, which has two alpha and two beta polypeptide subunits. Fetal hemoglobin concentrations can be elevated (usually above 0.5%) in children and adults affected by LEUKEMIA and several types of ANEMIA.	Hemoglobin F,Hemoglobin, Fetal
D006441	Hemoglobin A	Normal adult human hemoglobin. The globin moiety consists of two alpha and two beta chains.
D006443	Hemoglobin A2	An adult hemoglobin component normally present in hemolysates from human erythrocytes in concentrations of about 3%. The hemoglobin is composed of two alpha chains and two delta chains. The percentage of HbA2 varies in some hematologic disorders, but is about double in beta-thalassemia.	A2, Hemoglobin
D006455	Hemoglobins, Abnormal	Hemoglobins characterized by structural alterations within the molecule. The alteration can be either absence, addition or substitution of one or more amino acids in the globin part of the molecule at selected positions in the polypeptide chains.	Abnormal Hemoglobins
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man