Biomaterial Database

Formation of cyclic adenosine-3'-5'-monophosphate (cAMP) from adenosine-5'-phosphosulfate (APS) in Chlamydomonas reinhardtii CW 15? 1980

F Kühlhorn, and A Schmidt

Levels of cyclic adenosine monophosphate (cAMP) have been determined in the green alga Chlamydomonas reinhardtii strain CW 15. In the early exponential growth no cAMP was detected, however, in the middle of the exponential growth phase 2.4 pmol cAMP/mg protein were found and the cAMP level increased to 18.8 pmol/mg protein in the stationary phase. An enzyme fraction has been isolated from Chlamydomonas, which produced cAMP in vitro using adenosine-5'-phosphosulfate (APS) as a substrate. This protein fraction was isolated by ammoniumsulfate precipitation, DEAE-cellulose chromatography and Sephadex-G-100 gel filtration. A solvent system was developed for thin layer chromatography to separate cAMP from APS, adenosine-5'-phosphoramidat (APN), 5'-AMP and adenosine. The product was identified as cAMP by cochromatography, coelectrophoresis, treatment with phosphodiesterase, chromatography on Biorad-columns and by the protein-binding assay. The so far purified protein fraction produced 151 pmol cAMP/mg protein and hour in vitro, using APS as a substrate.

UI	MeSH Term	Description	Entries
D002696	Chlamydomonas	A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.	Chlamydomona
D000242	Cyclic AMP	An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.	Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000250	Adenosine Phosphosulfate	5'-Adenylic acid, monoanhydride with sulfuric acid. The initial compound formed by the action of ATP sulfurylase on sulfate ions after sulfate uptake. Synonyms: adenosine sulfatophosphate; APS.	Adenylyl Sulfate,Adenosine 5'-Phosphosulfate,Phospho-Adenylsulfate,5'-Phosphosulfate, Adenosine,Adenosine 5' Phosphosulfate,Phospho Adenylsulfate,Phosphosulfate, Adenosine,Sulfate, Adenylyl
D013429	Sulfatases	A class of enzymes that catalyze the hydrolysis of sulfate ESTERS.	Sulfatase
D013806	Theophylline	A methyl xanthine derivative from tea with diuretic, smooth muscle relaxant, bronchial dilation, cardiac and central nervous system stimulant activities. Theophylline inhibits the 3',5'-CYCLIC NUCLEOTIDE PHOSPHODIESTERASE that degrades CYCLIC AMP thus potentiates the actions of agents that act through ADENYLYL CYCLASES and cyclic AMP.	1,3-Dimethylxanthine,3,7-Dihydro-1,3-dimethyl-1H-purine-2,6-dione,Accurbron,Aerobin,Aerolate,Afonilum Retard,Aquaphyllin,Armophylline,Bronchoparat,Bronkodyl,Constant-T,Elixophyllin,Euphylong,Glycine Theophyllinate,Lodrane,Monospan,Nuelin,Nuelin S.A.,Quibron T-SR,Slo-Phyllin,Somophyllin-T,Sustaire,Synophylate,Theo Von Ct,Theo-24,Theo-Dur,Theobid,Theocin,Theoconfin Continuous,Theodur,Theolair,Theolix,Theon,Theonite,Theopek,Theophylline Anhydrous,Theophylline Sodium Glycinate,Theospan,Theostat,Theovent,Uniphyl,Uniphyllin,Uniphylline,1,3 Dimethylxanthine,Anhydrous, Theophylline,Constant T,ConstantT,Ct, Theo Von,Glycinate, Theophylline Sodium,Quibron T SR,Quibron TSR,Slo Phyllin,SloPhyllin,Sodium Glycinate, Theophylline,Somophyllin T,SomophyllinT,Theo 24,Theo Dur,Theo24,Theophyllinate, Glycine,Von Ct, Theo
D015056	1-Methyl-3-isobutylxanthine	A potent cyclic nucleotide phosphodiesterase inhibitor; due to this action, the compound increases cyclic AMP and cyclic GMP in tissue and thereby activates CYCLIC NUCLEOTIDE-REGULATED PROTEIN KINASES	3-Isobutyl-1-methylxanthine,Isobutyltheophylline,IBMX,1 Methyl 3 isobutylxanthine,3 Isobutyl 1 methylxanthine