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The peroxidase and immunofluorescent localization patterns of androgen-binding protein (ABP), a biological marker of Sertoli cell function, have been examined in cultured Sertoli cells isolated from 20- to 22-day-old rats. ABP immunoreactivity in the form of cytoplasmic granules of variable diameter was observed in Sertoli cells with characteristic lipid droplets and a colony-forming, epithelial-like growth pattern. Incubation of cultures with [3H]thymidine demonstrated that Sertoli cells continue to produce ABP while retaining their capability for synthesizing DNA and undergoing mitosis. A variable number of cultured Sertoli cells became morphologically transformed after exposure to follitropin (follicle-stimulating hormone) and pharmacological agents acting on cyclic nucleotide metabolism. The induced change in Sertoli cell shape coincided with a disappearance of ABP-containing granules from the cytoplasm. These observations demonstrate that localization of ABP by immunological techniques is a valuable tool for the characterization of structural and functional properties of Sertoli cell in culture.
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
December 1981,
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A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
December 1983,
Endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
January 1974,
Current topics in molecular endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
March 1979,
Acta endocrinologica,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
April 1981,
Endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
March 1982,
Experientia,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
March 1987,
Endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
January 1987,
Endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
February 1979,
Endocrinology,
A L Kierszenbaum, and
M Feldman, and
O Lea, and
W A Spruill, and
L L Tres, and
P Petrusz, and
F S French
October 1992,
The Journal of steroid biochemistry and molecular biology,
