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Persistent infection of mouse tumor cells with mumps virus. 1980

T Ogino, and S Kotake, and T O Yoshida

A mouse tumor cell line (MCT) persistently infected with mumps virus (strain Urabe) was obtained. No antibody or chemicals were required for establishment or maintenance of the infected cell line (M-MCT). No difference was observed by light microscopy between MCT and M-MCT cells, except for cytoplasmic inclusion bodies in 10--20% of the M-MCT cells. Hemadsorption of chicken erythrocytes, which did not necessarily coincide with inclusion positive cells was shown by 10--20% of the M-MCT cells. Immunofluorescence staining revealed that almost all M-MCT cells contained mumps virus antigens, which were seen mainly in the cytoplasm as fine granules, dots, or large clumps. Infectious mumps virus was consistently detected in the culture fluid and the released virus showed some temperature sensitivity when assayed at 34 degrees C and 40 degrees C. M-MCT was resistant to superinfection with homologous virus and showed some resistance to heterologous viruses. Thirty one clones of M-MCT were isolated by the soft agar method. Some, but not all, clones had viral antigen and all those with antigen released virus into the culture medium. The growth rates of MCT and M-MCT cells as monolayer cultures in vitro were similar, but the transplantability of M-MCT cells in syngeneic C57BL/6 mice was lower than that of MCT cells.

UI MeSH Term Description Entries
D007181 Inclusion Bodies, Viral An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies. Negri Bodies,Viral Inclusion Bodies,Negri Body,Bodies, Negri,Bodies, Viral Inclusion,Body, Negri,Body, Viral Inclusion,Inclusion Body, Viral,Viral Inclusion Body
D007372 Interferons Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions. Interferon
D009109 Mumps virus The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection. Epidemic Parotitis Virus,Myxovirus parotitidis,Epidemic Parotitis Viruses,Mumps viruses
D009374 Neoplasms, Experimental Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms. Experimental Neoplasms,Experimental Neoplasm,Neoplasm, Experimental
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D006382 Hemadsorption A phenomenon manifested by an agent or substance adhering to or being adsorbed on the surface of a red blood cell, as tuberculin can be adsorbed on red blood cells under certain conditions. (Stedman, 25th ed) Hemadsorptions
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral

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