-transparent.png){kind=logo}
Interferon was induced in leukocyte suspensions from human buffy coats by exposure to phytohemagglutinin P, concanavalin A (Con A) and staphylococcal enterotoxin A, under a variety of cell culture conditions. Con A was found to rapidly (within 12 h) induce high yields of antiviral activity (1.5 units/1000 cells). Lesser yields were obtained with the other two mitogens studied. The interferon was partially purified to a spec. act. around 10(5.3) units/mg protein, by batch adsorption on controlled-pore glass (CPG) beads and desorption by ethylene glycol. This material was characterized as containing mainly gamma-type interferon. Specifically on gel filtration, a fraction of 45000 daltons was obtained which could account for virtually all antiviral activity present in the starting material. Furthermore, the ethylene glycol-eluted antiviral activity was acid-labile, serologically distinct from alpha and beta-type interferon and strictly species-specific (no activity detectable on any of the available cell species sensitive to alpha and beta-type interferon). The crude culture supernatant also contained some antiviral activity which resembled beta-type interferon in that it adsorbed to CPG, could be desorbed by pH 2 buffer, was acid-resistant and could be neutralized by a specific anti-fibroblast interferon antiserum. The CPG/ethylene glycol-purified gamma-type interefron preparation was found to inhibit the growth of certain lymphoblastoid cells (Daudi and Molt-4). It also potentiated natural killer activity of fresh donor lymphocytes. In both respects, the gamma-type interferon preparation was not significantly more active than preparations of alpha and beta-interferon of similar antiviral potency.
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
January 1984,
International archives of allergy and applied immunology,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
January 1981,
Methods in enzymology,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
January 1986,
Methods in enzymology,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
November 1979,
Proceedings of the National Academy of Sciences of the United States of America,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
July 1967,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
March 1986,
Acta virologica,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
August 1986,
Immunology,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
October 1977,
The Journal of biological chemistry,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
March 1981,
Proceedings of the National Academy of Sciences of the United States of America,
M de Ley, and
J van Damme, and
H Claeys, and
H Weening, and
J W Heine, and
A Billiau, and
C Vermylen, and
P de Somer
December 1990,
Acta virologica,
