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5-Hydroxyindole-3-acetic acid has been determined at normal and increased levels in urine. The urine samples were filtered or centrifuged and then injected into a liquid chromatograph containing a reversed phase column with tributyl phosphate as stationary liquid phase and an aqueous buffer + methanol as eluent. 5-Hydroxyindole-3-acetic acid is detected in the eluate by a fluorometric detector coupled to the outlet of the separation column and the quantitation is performed by measurement of the peak heights of the sample and external standards. The percentage recovery was 97 and the precision 0.01 (sr). The method is suitable for the determination of increased levels of 5-hydroxyindole-3-acetic acid in urine from patients suffering from carcinoid tumours. Indole-3-acetic acid can be determined simultaneously.
K G Wahlund, and
B Edlén
July 1983,
Clinical chemistry,
K G Wahlund, and
B Edlén
January 1981,
Clinical chemistry,
K G Wahlund, and
B Edlén
November 1984,
Journal of chromatography,
K G Wahlund, and
B Edlén
November 2002,
Clinical chemistry,
K G Wahlund, and
B Edlén
August 1986,
Clinical chemistry,
K G Wahlund, and
B Edlén
March 1980,
Journal of chromatography,
K G Wahlund, and
B Edlén
July 1991,
Analytical biochemistry,
K G Wahlund, and
B Edlén
July 1992,
Clinica chimica acta; international journal of clinical chemistry,
K G Wahlund, and
B Edlén
March 1987,
Journal of chromatography,
K G Wahlund, and
B Edlén
June 1983,
Journal of chromatography,
