-transparent.png){kind=logo}
Synthesis of globin mRNA in erythroid spleen cells from anemic mice was measured after in vitro incubation under conditions in which the level of intracellular heme was manipulated. This newly synthesized globin mRNA was isolated by hybridization with globin cDNA covalently bound to cellulose. Isonicotinic acid hydrazide (INH) and penicillamine were used as specific inhibitors of heme synthesis. It has been found that a 120-min incubation of spleen erythroid cells with 5mM INH or 5mM penicillamine reduced [3H] uridine incorporation into globin mRNA by 24% or 36%, respectively. The addition of heme to INH- or penicillamine-treated cells almost completely restored [3H] uridine incorporation into globin mRNA. These results indicate that heme stimulates transcription of processing of globin mRNA.
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
January 1981,
Acta biologica et medica Germanica,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
December 1972,
Proceedings of the National Academy of Sciences of the United States of America,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
December 1972,
Proceedings of the National Academy of Sciences of the United States of America,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
January 1974,
Transactions of the Association of American Physicians,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
April 2017,
Human gene therapy methods,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
July 1978,
Blood,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
August 1975,
The Journal of biological chemistry,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
January 1964,
Progress in hematology,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
January 1977,
Proceedings of the National Academy of Sciences of the United States of America,
O Fuchs, and
P Ponka, and
J Borova, and
J Neuwirt, and
M Travnicek
January 1983,
Journal of cellular biochemistry,
