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Qualitative and quantitative changes of non-histone chromatin proteins of spleen cells during the primary immune response to sheep red blood cells and aggregated human gamma-globulin were described. Synthesis of non-histone chromatin proteins was measured by labelling with 3H-tryptophan during culture of spleen cells in vitro. Chromatin was isolated and labelled proteins were analysed by polyacrylamide gel electrophoresis. During the immune response to both antigens in chromatin of spleen cells three new fractions of non-histone chromatin proteins were synthesized: fractions F1 - M = 12 000 and H - M = 3 000, specific for sheep red blood cells and fractions I1 - M less than 3 000 and B - M = 120 000, specific for human gamma-globulin. The third antigen-non-specific fraction was synthesized at the time when the primary immune reaction was finishing. These new fractions were synthesized only in one of the analysed subpopulations of spleen cells. In thymocytes (non-fully-differentiated lymphoid cells) all these fractions were absent. Changes of non-histone chromatin proteins in thymocytes during the immune response were similar to those found during stimulation to proliferation by phytohemagglutinin in vitro.
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
June 1977,
Molecular biology reports,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
January 1976,
Acta biochimica Polonica,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
March 1975,
Biochemical and biophysical research communications,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
January 1983,
Molekuliarnaia biologiia,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
August 1981,
Molecular biology reports,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
January 1976,
Nauchnye doklady vysshei shkoly. Biologicheskie nauki,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
March 1972,
FEBS letters,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
March 1974,
Experimental cell research,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
May 1976,
Biochemical and biophysical research communications,
A Horst, and
E M Rakowicz-Szulczyńska, and
E Wiland
September 1972,
The Biochemical journal,
