Biomaterial Database

A chromogenic assay for evaluation of alpha 2-macroglobulin level in serum and cerebrospinal fluid. 1981

W Cullmann, and W Dick

A photometric assay using the chromogenic substrate carbobenzoxy-valyl-glycyl-arginine-p-nitroanilide acetate (CHROMOZYM TRY) for the determination of alpha 2-macroglobulin which binds trypsin as a trypsin-protein complex, thus preserving its amidolytic activity, even in the presence of aprotinin, has been studied in serum as well as in cerebrospinal fluid (CSF). Normal range in sera was found to be mean = 10.2 +/- 3.3 kU/l (male), mean = 10.9 +/- 3.2 kU/l (female), and mean = 11.9 +/- 5.0 U/l in CSF. Within-run precision in sera was ascertained to be C.V. = 2.06%, and in CSF C.V. = 2.15%, respectively. There was a good correlation with immunochemical methods: r = 0.974 in 60 sera (p less than 0.001) and r = 0.976 in 16 pathological samples of CSF (p less than 0.001).

UI	MeSH Term	Description	Entries
D002863	Chromogenic Compounds	Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).	Chromogenic Compound,Chromogenic Substrate,Chromogenic Substrates,Compound, Chromogenic,Compounds, Chromogenic,Substrate, Chromogenic,Substrates, Chromogenic
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000511	alpha-Macroglobulins	Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.	Slow alpha 2-Macroglobulins,alpha 2-Acute Phase Globulins,alpha-Macrofetoproteins,45S RNP,Acute-Phase alpha 1-Protein,Slow alpha 2-Globulin,alpha 1-Acute Phase Globulin,alpha 1-Acute Phase Protein,alpha 1-Macroglobulin,alpha 2-Acute Phase Globulin,alpha-Macrofetoprotein,Acute Phase alpha 1 Protein,RNP, 45S,Slow alpha 2 Globulin,Slow alpha 2 Macroglobulins,alpha 1 Acute Phase Globulin,alpha 1 Acute Phase Protein,alpha 1 Macroglobulin,alpha 1-Protein, Acute-Phase,alpha 2 Acute Phase Globulin,alpha 2 Acute Phase Globulins,alpha 2-Globulin, Slow,alpha 2-Macroglobulins, Slow,alpha Macrofetoprotein,alpha Macrofetoproteins,alpha Macroglobulins