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In 3- to 4-day embryonic chick limb buds, a region at the posterior margin of the limb, the zone of polarizing activity, appears to be responsible for signalling positional information along the anteroposterior axis. Our experiments were designed to test which biosynthetic processes are required for polarizing activity. We have treated polarizing regions with biochemical inhibitors, and then assayed their abilities to induce limb reduplications when grafted into anterior sites on host limb buds and also measured their capacities for protein, RNA, and DNA synthesis. DNA synthesis, and possibly oxidative phosphorylation, do not seem to be required for polarizing activity. But, glycolysis and protein and RNA synthesis are necessary, although not sufficient, for polarizing region activity. Activity seems particularly sensitive to inhibitors (actinomycin D and alpha-amanitin) of RNA synthesis.
L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
February 1976,
Nature,
L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
June 1987,
Development (Cambridge, England),
L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
June 1986,
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L S Honig, and
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A Hornbruch, and
L Wolpert
January 1983,
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L S Honig, and
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L Wolpert
June 1975,
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L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
December 1980,
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L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
June 1985,
Journal of embryology and experimental morphology,
L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
March 1975,
Nature,
L S Honig, and
J C Smith, and
A Hornbruch, and
L Wolpert
January 1988,
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L S Honig, and
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A Hornbruch, and
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