Biomaterial Database

Tissue concentration and release of substance P-like immunoreactivity in the dental pulp. 1981

E Brodin, and B Gazelius, and L Olgart, and G Nilsson

The concentration of substance P-like immunoreactivity (SPLI) was determined in dental pulps from cat, dog and man by radioimmunoassay. Pulps from mature cats showed the highest SPLI-levels (mean 32 pmol/g); these are comparable to or higher than those found in any other organ outside the central nervous system. The SPLI concentrations were lower in pulp from immature teeth. The immunological and chromatographic behaviour of the pulpal SPLI from cat and man resembled that of synthetic substance P (SP), indicating that the assayed substance is closely related to or identical with SP. Release of pulpal SPLI in canine teeth was evoked by stimulation of the inferior alveolar nerve in anaesthetized cats. In 9 of 16 experiments the amounts of SPLI found in pulp suprafusates following stimulation (4-31 fmol) were larger than those in unstimulated controls (0-7 fmol). Stimulation also reduced the pulpal tissue concentration of SPLI to 60% of that in homologous control pulps. The high concentration of SPLI found in the dental pulp and the demonstration of nerve-induced release strengthen the hypothesis that SP serves some function in the dental pulp.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010525	Peripheral Nerves	The nerves outside of the brain and spinal cord, including the autonomic, cranial, and spinal nerves. Peripheral nerves contain non-neuronal cells and connective tissue as well as axons. The connective tissue layers include, from the outside to the inside, the epineurium, the perineurium, and the endoneurium.	Endoneurium,Epineurium,Perineurium,Endoneuriums,Epineuriums,Nerve, Peripheral,Nerves, Peripheral,Perineuriums,Peripheral Nerve
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D003782	Dental Pulp	A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)	Dental Pulps,Pulp, Dental,Pulps, Dental
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man