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Several plasmids have been constructed which direct the synthesis of hepatitis B virus surface antigens in Escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. When injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. Either the NH2-terminal segment or the COOH-terminal segment of the surface antigens alone was sufficient to elicit the immune response, but antibodies against the two segments showed different specificities. The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 1981,
Progress in medical virology. Fortschritte der medizinischen Virusforschung. Progres en virologie medicale,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
August 1980,
Nature,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 2021,
Methods in molecular biology (Clifton, N.J.),
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
October 1984,
Gene,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 1988,
Doklady Akademii nauk SSSR,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
June 2000,
International archives of allergy and immunology,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
September 1979,
Infection and immunity,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 1985,
Gene,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 1983,
Doklady Akademii nauk SSSR,
P MacKay, and
M Pasek, and
M Magazin, and
R T Kovacic, and
B Allet, and
S Stahl, and
W Gilbert, and
H Schaller, and
S A Bruce, and
K Murray
January 1982,
Annali Sclavo; rivista di microbiologia e di immunologia,
