Biomaterial Database

In vitro effects of two immunopotentiators, Isoprinosine and NPT 15392, on murine T-cell differentiation and function. 1981

S Ikehara, and J W Hadden, and R A Good, and D G Lunzer, and R N Pahwa

A new immunopotentiator, NPT 15392, was analyzed in vitro for its capacity to induce a T-cell marker (Thy-1 antigen) and to enhance the mitogen-induced lymphocyte proliferation. The activity was compared with that of Isoprinosine and a putative thymic hormone (thymosin Fr. V). Thymic hormone preparation (thymosin Fr. V) and the non-thymic agents (Isoprinosine and NPT 15392) induced significant percentages (20-30%) of Thy-1 positive cells in nylon wool-nonadherent nu/nu spleen cells. Maximum induction was observed the concentrations of 100 microgram/ml thymosin Fr. V, 1.0 microgram/ml Isoprinosine, and 0.1 microgram/ml NPT 15392. In addition, NPT 15392 or Isoprinosine significantly augmented proliferation of C57BL/6J spleen cells stimulated by the T-cell mitogens (Con A and PHA) and B-cell mitogen (LPS). The effect of both agents was greater on spleen cells from aged mice (18 mth) than on young mice (3 mth). The minimum effective concentration of NPT was lower than that of Isoprinosine. The data indicate that Isoprinosine and NPT 15392 have actions on precursor T cells, mature T cells, and probably B cells.

UI	MeSH Term	Description	Entries
D007042	Hypoxanthines	Purine bases related to hypoxanthine, an intermediate product of uric acid synthesis and a breakdown product of adenine catabolism.
D007288	Inosine	A purine nucleoside that has hypoxanthine linked by the N9 nitrogen to the C1 carbon of ribose. It is an intermediate in the degradation of purines and purine nucleosides to uric acid and in pathways of purine salvage. It also occurs in the anticodon of certain transfer RNA molecules. (Dorland, 28th ed)
D007542	Inosine Pranobex	An alkylamino-alcohol complex of inosine used in the treatment of a variety of viral infections. Unlike other antiviral agents, it acts by modifying or stimulating cell-mediated immune processes rather than acting on the virus directly.	Inosiplex,Methisoprinol,Imunovir,Isoprinosin,Isoprinosine,Methysoprinol,Metisoprinol,NPT-10381,NPT 10381,NPT10381,Pranobex, Inosine
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008819	Mice, Nude	Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.	Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D008934	Mitogens	Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)	Mitogen,Phytomitogen,Phytomitogens
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D000276	Adjuvants, Immunologic	Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.	Immunoactivators,Immunoadjuvant,Immunoadjuvants,Immunologic Adjuvant,Immunopotentiator,Immunopotentiators,Immunostimulant,Immunostimulants,Adjuvant, Immunologic,Adjuvants, Immunological,Immunologic Adjuvants,Immunological Adjuvant,Adjuvant, Immunological,Immunological Adjuvants
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia