Biomaterial Database

Fetal hemoglobin biosynthesis in clonal cell culture. 1980

M Ogawa, and P N Porter

Availability of the clonal erythropoietic cell culture methods provided a unique opportunity to study human hemoglobin biosynthesis under controlled conditions. Observations in several laboratories during the last four years fundamentally agree and can be summarized as follows: (1) Biosynthesis of fetal hemoglobin (HbF) is markedly augmented in cultures of adult erythropoietic precursors. (2) HbF biosynthetic capabilities inversely parallel the maturational stages of adult erythropoietic precursors in the marrow. (3) Adult circulating precursors (BFU-e) are also capable of augmented HbF biosynthesis in culture. (4) The pattern of Hb synthesized by fetal erythropoietic precursors corresponds very closely to the Hb compositions in the mature erythrocytes of the donors. (5) Burst-promoting activities (BPA) rather than erythropoietin (Ep) appear to stimulate HbF biosynthesis by augmentation of proliferation of precursors which are programmed to produce F cells in vivo. (6) Individual BFU-e are heterogeneously committed to HbF biosynthesis and their frequency distributions reveal normal distributions. (7) Increase in HbF production in culture is primarily due to increased production of the number of cells containing Hbf. (8) Commitment to produce HbF continues during subcolony formation in culture. (9)HbF synthesized in culture of adult and umbilical cord blood BFU-e reveal "adult" and "intermediate" values of G gamma:A gamma ratios, respectively. (1)) Frequency distributions of individual bursts varying in relative G gamma synthesis (G gamma/[G gamma+A gamma]) reveal unimodal distributions. (11) Positive correlations exist between gamma/(gamma+beta) and G gamma/(G gamma + A gamma) values in individual bursts.

UI	MeSH Term	Description	Entries
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002999	Clone Cells	A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)	Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D004920	Erythropoiesis	The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.	Erythropoieses
D005260	Female		Females
D005312	Fetal Blood	Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.	Cord Blood,Umbilical Cord Blood,Blood, Cord,Blood, Fetal,Blood, Umbilical Cord,Bloods, Cord,Bloods, Fetal,Bloods, Umbilical Cord,Cord Blood, Umbilical,Cord Bloods,Cord Bloods, Umbilical,Fetal Bloods,Umbilical Cord Bloods
D005319	Fetal Hemoglobin	The major component of hemoglobin in the fetus. This HEMOGLOBIN has two alpha and two gamma polypeptide subunits in comparison to normal adult hemoglobin, which has two alpha and two beta polypeptide subunits. Fetal hemoglobin concentrations can be elevated (usually above 0.5%) in children and adults affected by LEUKEMIA and several types of ANEMIA.	Hemoglobin F,Hemoglobin, Fetal
D005914	Globins	A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure.	Globin