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Recently, we published an assembly map of the 50S ribosomal subunit from E. coli, incorporating 23S and 5S RNA and 26 out of the 32 L-proteins (Röhl and Nierhaus (1982) Proc. Natl. Acad. Sci. U.S.A., in press). In this paper we present the experimental details which led to the compilation of this 50S assembly map. The following experimental strategy was pursued: In order to elucidate binding of a protein to 23S RNA or a protein-protein interdependence, 23S rRNA and a set of purified ribosomal proteins were incubated under reconstitution conditions, the RNA-protein complex was separated from the non-bound proteins by sucrose gradient centrifugation and the proteins contained in the complex were determined by means of gel electrophoresis.
R Röhl, and
H E Roth, and
K H Nierhaus
February 1982,
Proceedings of the National Academy of Sciences of the United States of America,
R Röhl, and
H E Roth, and
K H Nierhaus
December 1982,
Proceedings of the National Academy of Sciences of the United States of America,
R Röhl, and
H E Roth, and
K H Nierhaus
July 2001,
Biochimica et biophysica acta,
R Röhl, and
H E Roth, and
K H Nierhaus
December 1975,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
R Röhl, and
H E Roth, and
K H Nierhaus
June 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R Röhl, and
H E Roth, and
K H Nierhaus
January 1992,
The EMBO journal,
R Röhl, and
H E Roth, and
K H Nierhaus
March 1980,
Journal of molecular biology,
R Röhl, and
H E Roth, and
K H Nierhaus
July 1976,
Proceedings of the National Academy of Sciences of the United States of America,
R Röhl, and
H E Roth, and
K H Nierhaus
January 1970,
Biochemical and biophysical research communications,
R Röhl, and
H E Roth, and
K H Nierhaus
February 1978,
Biochemistry,
