-transparent.png){kind=logo}
Simian virus 40 large tumor antigen was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified large tumor antigen, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP, and UMP. Furthermore, RNA fragments of approximately 45 nucleotides could be isolated from large tumor antigen purified by the same procedure. Mapping of the T1 oligonucleotides showed a high complexity, as indicated by the presence of unique sequences of 15-30 nucleotides and of poly(A). This is compatible with the hypothesis that these RNA fragments are derived from cellular pre-mRNAs or mRNAs. Our results suggest that Simian virus 40 large tumor antigen is a RNA-binding protein and might possibly be involved in regulation of synthesis, maturation, or translation of cellular mRNAs.
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
October 1987,
Biochimica et biophysica acta,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
November 1991,
Biochimica et biophysica acta,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
May 1979,
Journal of virology,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
December 1984,
Biochemistry,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
September 1984,
Proceedings of the National Academy of Sciences of the United States of America,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
May 1994,
Biochimica et biophysica acta,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
March 1985,
Virology,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
January 1992,
Annual review of biochemistry,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
March 1986,
Journal of virology,
E W Khandjian, and
M Loche, and
J L Darlix, and
R Cramer, and
H Türler, and
R Weil
April 1979,
The Journal of biological chemistry,
