-transparent.png){kind=logo}
The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. It was found that the rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) was found to have the highest rate of addition. Low salt and temperature produced maximal incorporation.
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
January 1997,
Photochemistry and photobiology,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
August 1983,
Biochemistry,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
March 1982,
Biochemistry,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
October 1970,
Photochemistry and photobiology,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
November 1988,
Nucleic acids research,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
January 1986,
IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
November 1985,
Proceedings of the National Academy of Sciences of the United States of America,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
May 1979,
Biochemistry,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
January 1992,
Journal of photochemistry and photobiology. B, Biology,
J F Thompson, and
J P Bachellerie, and
K Hall, and
J E Hearst
June 1981,
Biochemistry,
