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A spectrofluorometric method to determine 5-HT and 5-HIAA in rat diencephalon has been developed, following the criterion of unifying the methodology in determining biogenic amines and their metabolites. Linearity in the method remains in the interval between 0.01 microgram and 0.05 microgram. Recovery is about 70% for amine and about 80% for the metabolite. Highest concentrations of 5-HT appear in one month old female animals, and are significantly higher than those found in the male population of the same age. Concentrations in two month old animals decrease, without significant differences between males and females. Values found for 5-HIAA seem not to change so significantly, although a slight decrease is observed in the elder males.
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
July 1970,
British journal of pharmacology,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
January 1974,
Acta psychiatrica Scandinavica,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
May 1983,
Canadian journal of physiology and pharmacology,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
March 1972,
Analytical biochemistry,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
December 1982,
Neuroendocrinology,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
November 1975,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
January 1975,
Roczniki Panstwowego Zakladu Higieny,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
October 1968,
Lancet (London, England),
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
January 1995,
Biomedical chromatography : BMC,
E Martínez-Conde, and
M J López-Puente, and
M L Leret, and
A Fraile
January 2001,
Neuropsychobiology,
