Biomaterial Database

Rapid phenotyping of the group specific component by immunofixation on cellulose acetate. 1977

B W Grunbaum, and P L Zajac

Determination of the genetically controlled variants of the polymorphic Gc system was achieved by electrophoresis on cellulose acetate membranes followed by immunofixation with a specific anti-Gc antiserum. The method is applicable to plasma, whole hemolyzed blood, and dried blood. Multiple specimens can be analyzed simultaneously within 60 to 80 min. The cellulose acetate electrophoretogram of the Gc variants remains as a permanent record.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D001789	Blood Group Antigens	Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.	Blood Group,Blood Group Antigen,Blood Groups,Antigen, Blood Group,Antigens, Blood Group,Group Antigen, Blood,Group, Blood,Groups, Blood
D001801	Blood Stains	Antigenic characteristics and DNA fingerprint patterns identified from blood stains. Their primary value is in criminal cases.	Blood Stain,Stain, Blood,Stains, Blood
D004586	Electrophoresis	An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.	Electrophoreses
D004588	Electrophoresis, Cellulose Acetate	Electrophoresis in which cellulose acetate is the diffusion medium.	Cellulose Acetate Electrophoreses,Cellulose Acetate Electrophoresis,Electrophoreses, Cellulose Acetate
D005554	Forensic Medicine	The application of medical knowledge to questions of law.	Legal Medicine,Medicine, Forensic,Medicine, Legal
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man