Biomaterial Database

Scanning electron microscopy of retinal ganglion cells by applying D,L-2-aminoadipic acid. 1982

M Wakakura, and S Ishikawa

Retinal ganglion cells of the cat were clearly demonstrated in scanning electron microscopy. For identification of the cells, D,L-2-aminoadipic acid, a gliotoxic compound, was applied. Müller cells as well as astrocytes were destroyed by intravitreal injection of the aminoadipic acid prior to the enucleation. Balloon-shaped retinal ganglion cells with an axonal process were visualized in the normal cat retina. Flattened and sometimes indented ganglion cells were observed in the pathologic retina which was made 2 weeks after optic nerve transection. Pretreatment with the aminoadipic acid was a very effective method for the identification of the retinal ganglion cells by scanning electron microscopy.

UI	MeSH Term	Description	Entries
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D012160	Retina	The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.	Ora Serrata
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D015074	2-Aminoadipic Acid	A metabolite in the principal biochemical pathway of lysine. It antagonizes neuroexcitatory activity modulated by the glutamate receptor, N-METHYL-D-ASPARTATE; (NMDA).	alpha-Aminoadipic Acid,2 Aminoadipic Acid,2-Aminohexanedioic Acid,2 Aminohexanedioic Acid,Acid, 2 Aminoadipic,Acid, 2-Aminoadipic,Acid, 2-Aminohexanedioic,Acid, alpha-Aminoadipic,Aminoadipic Acid, 2,alpha Aminoadipic Acid