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Nucleic acid and protein synthesis was determined in immature myeloid cells isolated from seven normal bone marrow samples and compared to blasts from nine patients with fullblown acute non-lymphocytic leukemia. Samples obtained from normal bone marrow contained a mean of 82% immature myeloid cells with 29.9% S-phase cells. These cells incorporated [3H]thymidine with a mean of 17,500 counts/min [3H]uridine with a mean of 24,000 counts/min and [3H]leucine with a mean of 2100 counts/min per 0.2 x 10(6) cells. Leukemic bone marrow cells could be separated in fractions with different proliferative activities. Leukemic samples with a mean of 3.6% S-phase cells incorporated [3H]thymidine with a mean of 1100 counts/min, [3H]uridine with a mean of 15,700 counts/min and ]3H]leucine with a mean of 2600 counts/min per 0.2 x 10(6) cells. For leukemic samples with a mean of 30.6% S-phase cells these values were: [3H]thymidine 22,200 counts/min, [3H]uridine 49,700 counts/min and [3H]leucine 6700 counts min per 0.2 x 10(6) cells. The incorporation studies were carried out for the first time in normal and leukemic cells with a comparable proliferative activity. Non-lymphocytic leukemic blastic cells showed a two-fold increase in RNA synthesis and a three-fold increase in protein synthesis compared to enriched samples of normal early myeloid cells with the same proliferative activity.
J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
January 1971,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
November 1957,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
July 1966,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
June 1975,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
April 1969,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
July 1961,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
February 1972,
Experimental cell research,
J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
May 1954,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
December 1969,
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J T Burghouts, and
A M Plas, and
M H Salden, and
H M Wessels
May 1973,
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