[Relationship between chlorophyll concentration and the energy reaction between protochlorophyll and chlorophyll in mixed associations]. 1977

E I Zen'kevich, and G A Kochubeev, and A P Losev, and G P Gurinovich

In the paper results are presented of investigation of protochlorophyll (PChl) and chlorophyll (Chl) mixed associations and of interaction between them within the polymer molecular complex, which forms in mixture of water-dioxane (1 : 4). The initial PChl concentration in all solutions was constant (CPChl = 1 . 10(-5) m/l), and Chl concentration varied from 1.10(-8) m/l up to 2.10(-5) m/l. It is shown that with the rise of Chl proportion in the mixed aggregate the rearrangement of both donor (PChl) and acceptor (Chl) components of complex takes place. The luminescence quenching of PChl and the sensitization of Chl emission in mixed pigment associates were investigated of different Chl levels and the evaluation of efficiency of intracomplex electronic excitation energy transfer, determined from quenching and sensibilization, was performed. Similar dependence of energy transfer effectiveness on Chl concentration, determined by the two above-mentioned methods shows that the excitation migration in an associate takes place without losses. An analysis of results permits to conclude that a small trapping efficiency of PChl excitation by the acceptor part of the complex may be connected with the existence of the prerelaxation reverse energy transfer from Chl to PChl in mixed pigment associates. On the basis of the obtained data a mechanism of energy transfer from protochlorophyllide to chlorophyllide in etiolated leaves and homogenates is discussed.

UI MeSH Term Description Entries
D011521 Protochlorophyllide A photo-active pigment localized in prolamellar bodies occurring within the proplastids of dark-grown bean leaves. In the process of photoconversion, the highly fluorescent protochlorophyllide is converted to chlorophyll. Protochlorophyllide A,MV-PChlide,Monovinyl Protochlorophyllide,MV PChlide,Protochlorophyllide, Monovinyl
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D002734 Chlorophyll Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms. Phyllobilins,Chlorophyll 740
D002942 Circular Dichroism A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Circular Dichroism, Vibrational,Dichroism, Circular,Vibrational Circular Dichroism
D004146 Dioxanes Compounds that contain the structure 1,4-dioxane.
D004735 Energy Transfer The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER. Transfer, Energy
D005454 Fluorescence Polarization Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. Anisotropy, Fluorescence,Fluorescence Anisotropy,Polarization, Fluorescence,Anisotropies, Fluorescence,Fluorescence Anisotropies,Fluorescence Polarizations,Polarizations, Fluorescence
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013057 Spectrum Analysis The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Spectroscopy,Analysis, Spectrum,Spectrometry

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