-transparent.png){kind=logo}
The cyclopeptide antibiotic gramicidin S or tyrocidine in concentrations of 2 to 4 mumol/mg of membrane protein inhibited the active transport of [3H]alanine and [3H]uridine in membrane vesicles isolated from Bacillus brevis and Bacillus subtilis. We used one analog of gramicidin S and two of tyrocidine A to study the relationship between peptide structure and antibacterial action as seen in inhibiting active transport and in vitro transcription and in delaying spore outgrowth. The data showed that [Ser2,2']-gramicidin S, in which the two ornithine residues were replaced by two serines, was at least 50 times less active antibacterially and gave a low response in transport inhibition and delay of spore outgrowth compared with the natural peptide. The antibacterial activity of [Val6]-tyrocidine A was twice lower than that of tyrocidine A, and it also showed a considerable reduction in transport and transcription inhibition. [Orn7]-tyrocidine A containing two ornithine residues in positions corresponding to those in gramicidin S was almost inactive in all functions tested. The correlation between peptide structure and activity is discussed.
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
August 1969,
The Journal of biological chemistry,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
November 1986,
Journal of biochemistry,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
January 1978,
Biochemical Society transactions,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
March 1997,
Acta crystallographica. Section D, Biological crystallography,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
January 1983,
Arzneimittel-Forschung,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
February 1982,
Journal of general microbiology,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
July 1955,
Nature,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
March 1960,
Science (New York, N.Y.),
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
August 2017,
Journal of medical microbiology,
W Danders, and
M A Marahiel, and
M Krause, and
N Kosui, and
T Kato, and
N Izumiya, and
H Kleinkauf
May 1983,
Journal of general microbiology,
