Biomaterial Database

Monoclonal antibodies against a plant virus. 1982

R G Dietzgen, and E Sander

Eight stable hybridoma clones derived from fusion of spleen cells of Tobacco Mosaic Virus (TMV) immunized mice with murine myeloma cells were grown in mass culture. They were obtained by 2 subsequent limiting dilution cloning cycles and subcultivation. Five of these clones secreted monoclonal antibodies which reacted with TMV nucleocapsid but not with capsid monomers and the monoclonal antibodies secreted by 3 other clones reacted with the TMV capsid monomer but not with the nucleocapsid. The specificity was determined by Enzyme Linked Immunosorbent Assay (ELISA) adjusted for the detection of antibodies in hybridoma culture supernatants.

UI	MeSH Term	Description	Entries
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914	Antibodies, Viral	Immunoglobulins produced in response to VIRAL ANTIGENS.	Viral Antibodies
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D014027	Tobacco Mosaic Virus	The type species of TOBAMOVIRUS which causes mosaic disease of NICOTIANA. Transmission occurs by mechanical inoculation.	Mosaic Virus, Tobacco,Mosaic Viruses, Tobacco,Tobacco Mosaic Viruses,Virus, Tobacco Mosaic,Viruses, Tobacco Mosaic
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus