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The nicotinic acetylcholine receptor (AChR) from the electroplax of the ray Torpedo californica is composed of five subunits present in a molar stoichiometry of alpha 2 beta gamma delta (refs 1-3) and contains both the binding site for the neurotransmitter and the cation gating unit (reviewed in refs 4-6). We have recently elucidated the complete primary structures of the alpha-, beta- and delta-subunit precursors of the T. californica AChR by cloning and sequencing cDNAs for these polypeptides. Here, we report the whole primary structure of the gamma-subunit precursor of the AChR deduced from the nucleotide sequence of the cloned cDNA. Comparison of the amino acid sequences of the four subunits reveals marked homology among them. The close resemblance among the hydrophilicity profiles and predicted secondary structures of all the subunits suggests that these polypeptides are oriented in a pseudosymmetric fashion across the membrane. Each subunit contains four putative transmembrane segments that may be involved in the ionic channel. The transmembrane topology of the subunit molecules has also been inferred.
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
May 1984,
Proceedings of the National Academy of Sciences of the United States of America,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
June 1983,
The Journal of biological chemistry,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
January 1979,
Biochemistry,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
January 1984,
Biochemical Society symposium,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
January 1979,
Advances in cytopharmacology,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
November 1977,
Journal of molecular biology,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
May 1979,
Biochemistry,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
July 1982,
Biochemistry,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
June 1986,
European journal of biochemistry,
M Noda, and
H Takahashi, and
T Tanabe, and
M Toyosato, and
S Kikyotani, and
Y Furutani, and
T Hirose, and
H Takashima, and
S Inayama, and
T Miyata, and
S Numa
May 1979,
FEBS letters,
