Biomaterial Database

The actions of tetraethylammonium on dispersed acini from guinea pig pancreas. 1983

K E McArthur, and R T Jensen, and J D Gardner

In dispersed acini from guinea pig pancreas, replacing extracellular sodium by tetraethylammonium (1) abolished carbamylcholine-stimulated amylase secretion but did not alter the increase in amylase secretion caused by the C-terminal octapeptide of cholecystokinin, bombesin, ionophore A23187, vasoactive intestinal peptide or 8-bromoadenosine 3':5' monophosphate, (2) caused a parallel rightward shift in the dose-response curve for carbamylcholine-stimulated amylase secretion and (3) inhibited binding of N-[3H]methyl scopolamine to muscarinic cholinergic receptors. Detectable inhibition of carbamylcholine-stimulated amylase secretion and binding of N-[3H]methyl scopolamine occurred with 300 microM tetraethylammonium, and half-maximal inhibition of these functions occurred with 1-2 mM tetraethylammonium. Replacing extracellular sodium by Tris did not alter the stimulation of enzyme secretion caused by any secretagogue tested. These results indicate that the tetraethylammonium is a muscarinic cholinergic receptor antagonist and that enzyme secretion from pancreatic acini does not depend on extracellular sodium.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010179	Pancreas	A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000681	Amylases	A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.	Diastase,Amylase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012602	Scopolamine Derivatives	Analogs or derivatives of scopolamine.	Scopolamines,Derivatives, Scopolamine
D013757	Tetraethylammonium Compounds	Quaternary ammonium compounds that consist of an ammonium cation where the central nitrogen atom is bonded to four ethyl groups.	Tetramon,Tetrylammonium,Compounds, Tetraethylammonium
D019832	N-Methylscopolamine	A muscarinic antagonist used to study binding characteristics of muscarinic cholinergic receptors.	DD-234,Hyoscine Methiodide,Hyoscine Methobromide,Methscopolamine,Methylscopolamine Nitrate,Methylscopolammonium Methylsulfate,N-Methylscine Methylsulfate,N-Methylscopolamine Bromide,N-Methylscopolamine Iodide,N-Methylscopolamine Methylchloride,N-Methylscopolamine Methylsulfate,N-Methylscopolamine Nitrate,Scopolamine Methylbromide,Skopyl,Ulix,Bromide, N-Methylscopolamine,DD 234,DD234,Iodide, N-Methylscopolamine,Methiodide, Hyoscine,Methobromide, Hyoscine,Methylbromide, Scopolamine,Methylchloride, N-Methylscopolamine,Methylsulfate, Methylscopolammonium,Methylsulfate, N-Methylscine,Methylsulfate, N-Methylscopolamine,N Methylscine Methylsulfate,N Methylscopolamine,N Methylscopolamine Bromide,N Methylscopolamine Iodide,N Methylscopolamine Methylchloride,N Methylscopolamine Methylsulfate,N Methylscopolamine Nitrate,Nitrate, Methylscopolamine,Nitrate, N-Methylscopolamine
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In