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A micro solid-phase radioimmunoassay (micro-SPRIA) was developed to demonstrate type-specific antibodies to herpes simplex virus types 1 and 2 (HSV1 and HSV2). Glycoproteins from the 123,000 dalton region of HSV1 (VP123) and the 119,000 dalton region of HSV2 (VP119) were isolated on preparative polyacrylamide gels for use as antigens in the micro-SPRIA. Human sera selected from clinical samples by virological history and appropriate microneutralization data were used to standardize the micro-SPRIA. Optimization of the assay required the use of siliconized microtiter wells for adsorption of antigen. Maximized results were highly dependent on the concentrations of antigen, primary antibody, and secondary antibody as well as the diluents used for these principal test reagents. Incorporation of HSV glycoproteins of each respective type with the optimal condition established in this study facilitates the direct detection of type-specific antibody in human sera.
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
January 1979,
Intervirology,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
October 1985,
Journal of virological methods,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
June 1979,
Infection and immunity,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
January 1976,
Vox sanguinis,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
August 1986,
Experimental parasitology,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
January 1981,
Journal of immunological methods,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
July 1977,
The Journal of infectious diseases,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
May 1969,
Immunochemistry,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
October 1975,
Journal of immunological methods,
D O Matson, and
K Adler-Storthz, and
E Adam, and
G R Dreesman
January 1983,
Medical laboratory sciences,
