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Recent studies have demonstrated that 5-fluoro-2'-deoxyuridine (FdUrd) misincorporates in eukaryotic DNA. We have extended these findings and studied the relationship between DNA incorporation of FdUrd and cell lethality. This required an approach that would distinguish the DNA and RNA effects of this agent. The specific effect of (FdUrd)DNA formation on L1210 clonogenic survival was, therefore, monitored using deoxyuridine (dUrd) to inhibit the metabolism and subsequent incorporation of FdUrd into RNA. The results demonstrated that misincorporation of FdUrd in DNA resulted in lethal cellular events. These observations suggest a new mechanism of action for this cytotoxic and mutagenic agent.
D W Kufe, and
P Scott, and
R Fram, and
P Major
September 1981,
The Journal of biological chemistry,
D W Kufe, and
P Scott, and
R Fram, and
P Major
September 1963,
Nature,
D W Kufe, and
P Scott, and
R Fram, and
P Major
September 1974,
Journal of medicinal chemistry,
D W Kufe, and
P Scott, and
R Fram, and
P Major
May 1981,
Antimicrobial agents and chemotherapy,
D W Kufe, and
P Scott, and
R Fram, and
P Major
January 1981,
Cancer chemotherapy and pharmacology,
D W Kufe, and
P Scott, and
R Fram, and
P Major
January 1986,
Advances in experimental medicine and biology,
D W Kufe, and
P Scott, and
R Fram, and
P Major
November 1982,
Molecular pharmacology,
D W Kufe, and
P Scott, and
R Fram, and
P Major
January 1985,
Biochemical and biophysical research communications,
D W Kufe, and
P Scott, and
R Fram, and
P Major
January 1990,
Anticancer research,
D W Kufe, and
P Scott, and
R Fram, and
P Major
September 1981,
Biochemical and biophysical research communications,
