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Monoclonal antibodies were obtained against the membrane-bound lysosomal enzyme beta-glucocerebrosidase (acid beta-glucosidase), which is deficient in Gaucher's disease. BALB/c mice were immunized with homogeneous enzyme protein extracted from a sodium dodecyl sulphate/polyacrylamide gel. The mice were subsequently hyperimmunized with partially purified enzyme prior to fusion of spleen cells with myeloma cells. After fusion, 32 primary hybrid cell populations were obtained which continued to produce antibodies against beta-glucocerebrosidase after prolonged time of culture. All antibodies reacted with both native and denatured enzyme. Four primary cell populations were subcloned and the antibodies produced were characterized. The antibodies were all four of the IgG1 subclass. Three of these antibodies bind to protein A whereas one does not. The results of binding assays indicated that three of the antibodies react with the same antigenic domain (epitope 1), but the fourth with a different one (epitope 2). Probably two antigenic determinants are present in epitope 1 since one of the antibodies with specificity for epitope 1 is inactivated after iodination by the chloramine-T procedure whereas a second one is not.
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
May 2005,
Neurobiology of aging,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
December 1988,
Biulleten' eksperimental'noi biologii i meditsiny,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
October 2002,
Hybridoma and hybridomics,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
October 1989,
Hybridoma,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
May 1998,
The Prostate,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
January 1995,
Upsala journal of medical sciences,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
June 1996,
In vitro cellular & developmental biology. Animal,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
September 1984,
Archives of pathology & laboratory medicine,
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
August 2009,
Hybridoma (2005),
R A Barneveld, and
F P Tegelaers, and
E I Ginns, and
P Visser, and
E A Laanen, and
R O Brady, and
H Galjaard, and
J A Barranger, and
A J Reuser, and
J M Tager
June 1987,
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
