-transparent.png){kind=logo}
The immune complexes formed by human growth hormone or human chorionic somatomammotropin and various monoclonal antibodies have been studied by gel filtration and polyacrylamide gel electrophoresis. Two of the monoclonal antibodies gave rise to complexes with molecular weights suggesting an antigen:antibody 1:1 ratio. When both antibodies were simultaneously incubated with human growth hormone the ratio estimated for the new complex was 1:2, indicating the existence of two nonoverlapping epitopes in the antigen. The other monoclonal antibodies exhibited a more intricate behavior: incubated separately with human growth hormone they gave rise to both types of the aforementioned complexes. A similar phenomenon could be demonstrated with human chorionic somatomammotropin. The study of the immunoreactivity of a synthetic peptide indicates that the involved epitopes are localized within the region limited by amino acid residues 44 and 128 of human growth hormone.
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
December 1984,
Journal of immunological methods,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
December 1982,
Molecular immunology,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
September 1979,
Proceedings of the National Academy of Sciences of the United States of America,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
April 1973,
Endocrinology,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
January 1971,
Experientia,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
May 1990,
The Biochemical journal,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
February 1980,
International journal of peptide and protein research,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
January 1972,
Obstetrics and gynecology annual,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
November 1974,
Archives of biochemistry and biophysics,
N Vita, and
E Poskus, and
C Penã, and
A R Pérez, and
A C Paladini
April 1971,
American journal of obstetrics and gynecology,
