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Monospecific anti-[rat alpha-foetoprotein(alpha-FP)] immunoglobulin G was coupled to CNBr-activated Sepharose-4B (4.5 mg/ml packed volume of gel) to yield an adsorbent. The immunoaffinity column was used to isolate alpha-FP from neonatal-rat skin. Purified skin alpha-FP was found to be immunologically and electrophoretically similar to serum alpha-FP. It yielded a single band with mol.wt. 68000 on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. However, on polyacrylamide-gel electrophoresis under non-denaturing conditions, the alpha-FP displayed slow- and fast-moving variants similar to those observed in serum alpha-FP. A Scatchard plot of oestradiol binding to the alpha-FP yielded an association constant of 2.5 X 10(9)M-1 by dextran-coated-charcoal and 0.75 X 10(8)M-1 by Sephadex-gel-filtration procedures respectively. Skin explants from newborn rats were found to incorporate [14C]leucine into immunoprecipitable intracellular alpha-FP. Cycloheximide inhibited the synthesis of alpha-FP in skin explant culture. Our results indicate that newborn-rat skin contains alpha-FP that is similar to serum alpha-FP and which may arise in neonatal-rat skin as a result of synthesis in situ.
K Mujoo, and
M Ali, and
M K Sahib
June 1971,
Clinica chimica acta; international journal of clinical chemistry,
K Mujoo, and
M Ali, and
M K Sahib
October 1978,
Archives internationales de physiologie et de biochimie,
K Mujoo, and
M Ali, and
M K Sahib
December 1980,
Journal of biochemistry,
K Mujoo, and
M Ali, and
M K Sahib
December 2001,
Brain research. Brain research protocols,
K Mujoo, and
M Ali, and
M K Sahib
June 1979,
The Biochemical journal,
K Mujoo, and
M Ali, and
M K Sahib
May 1984,
Acta endocrinologica,
K Mujoo, and
M Ali, and
M K Sahib
May 1969,
Biochemistry,
K Mujoo, and
M Ali, and
M K Sahib
December 1967,
Biochemistry,
K Mujoo, and
M Ali, and
M K Sahib
April 1974,
FEBS letters,
