-transparent.png){kind=logo}
The lysine-rich histones of chicken liver were studied in order to see whether a protein similar to mammalian histone H1o was present in this lower vertebrate. The following biochemical methods were used: sodium dodecylsulphate and acid-urea electrophoresis, gel exclusion chromatography on BioGel P100, and ion-exchange chromatography on BioRex 70. Specific polyclonal antibodies were elicited against purified mouse liver H1o and chicken erythrocyte H5, and applied for the further characterization of the chicken H1 subfractions obtained chromatographically. The results from microcomplement fixation and enzyme-linked immunosorbent assays showed that the presumptive chicken liver H1o shared common antigenic determinants with the mammalian H1o and the chicken liver H5. Based on the combined biochemical and immunological evidence, we conclude that an H1o-like protein is present in quiescent differentiated avian cells. The data of Smith et al. [34], who did not find this specific lysine-rich histone in resting chicken cells, are discussed.
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
December 1980,
Nucleic acids research,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
July 1984,
Molecular biology reports,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
January 1985,
Comparative biochemistry and physiology. B, Comparative biochemistry,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
January 1984,
Differentiation; research in biological diversity,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
June 1983,
Biochimica et biophysica acta,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
July 1983,
FEBS letters,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
October 1986,
European journal of biochemistry,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
April 1993,
Analytical biochemistry,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
February 1999,
Journal of theoretical biology,
L N Srebreva, and
N B Andreeva, and
K G Gasaryan, and
R G Tsanev, and
J S Zlatanova
February 1971,
Nature: New biology,
