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Thymic epithelial cells have a critical influence on T-cell differentiation. In order to characterize these cells in humans, a serum-free growth medium was developed for their long-term culture. Important components of this medium included transferrin, epidermal growth factor, prostaglandin E1, and selenious acid. The presence of a keratin cytoskeleton, tonofilaments, and desmosomes confirmed the epithelial nature of these cells. Indirect immunofluorescence study of these epithelial cells demonstrated the presence of Ia and B-2 microglobulin antigens. The availability of highly enriched thymic epithelial cultures should simplify the functional characterization of this cell.
I J Rimm, and
A K Bhan, and
E E Schneeberger, and
S F Schlossman, and
E L Reinherz
January 2024,
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A K Bhan, and
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S F Schlossman, and
E L Reinherz
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E L Reinherz
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E E Schneeberger, and
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E L Reinherz
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I J Rimm, and
A K Bhan, and
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E L Reinherz
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I J Rimm, and
A K Bhan, and
E E Schneeberger, and
S F Schlossman, and
E L Reinherz
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Differentiation; research in biological diversity,
