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A series of four monoclonal antibodies was raised against suspensions of normal adult WAB/Not rat hepatocytes. An immunoperoxidase-staining technique was used to examine the distribution of determinants detected by these antibodies on frozen sections of fetal, neonatal, adult, and regenerating liver and on a range of 4-dimethylaminoazobenzene-induced liver lesions, including a panel of 32 primary liver carcinomas. Three of the antibodies were directed against hepatocytes, while a fourth antibody stained stromal elements within the liver. The determinants detected by the anti-hepatocyte monoclonal antibodies arose in a specific sequence during normal liver development and, when assessed in conjunction, characterized several phenotypes associated with stages in normal hepatocyte differentiation. These same antibody-defined phenotypes were expressed by the primary liver carcinomas, and the distribution of phenotypes among the tumors revealed a heterogeneity which was not evident from a conventional morphological classification. Primary liver tumors expressed a total of four antibody-defined phenotypes, whereas gamma-glutamyl transpeptidase-positive foci of hepatocytes and neoplastic nodules expressed, respectively, only one or 2 antibody-defined phenotypes. We suggest that monoclonal antibodies directed against normal liver cell components may provide a means to establish lineage relationships between cell populations involved in hepatocarcinogenesis.
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
September 1985,
British journal of haematology,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
January 1979,
Proceedings of the National Academy of Sciences of the United States of America,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
March 1983,
Cancer research,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
October 1984,
American journal of clinical pathology,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
January 1986,
Anticancer research,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
January 1983,
Basic life sciences,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
January 1986,
Anticancer research,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
March 1981,
Diabetes,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
January 1978,
Current topics in microbiology and immunology,
C H Holmes, and
E B Austin, and
A Fisk, and
B Gunn, and
R W Baldwin
October 1989,
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
