The Shimadzu CS 920 densitometer: illumination of thin layer chromatograms with a parallel light beam and its effects on quantification. 1984

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The Shimadzu CS 920 has shown to be an easy to handle flying-spot densitometer that enables rapid quantification of thin layer chromatograms. Linear calibration curves with a relative standard deviation of 3 to 4% in the slope can be obtained for Sudan Yellow on Merck H60 plates. However, contrary to the statements in the instrument manual, the 'linearizer' performance is not in agreement with the Kubelka and Munk theory. Consequently, the CS 920 can only be used after empirically determined adjustment of the 'linearizer'. Furthermore, errors in analysis can increase up to 30% by using TLC-plates with fluctuating layer thickness.

UI MeSH Term Description Entries
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D003720 Densitometry The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material. Densitometries
D013194 Staining and Labeling The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts. Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
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