-transparent.png){kind=logo}
Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.
Related Publications
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
December 1984,
Nucleic acids research,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
January 1993,
Methods in molecular biology (Clifton, N.J.),
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
January 1979,
Methods in enzymology,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
May 1988,
Nucleic acids research,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
December 1984,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
January 1985,
Biochimie,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
September 2009,
Nucleosides, nucleotides & nucleic acids,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
March 1986,
Nucleic acids research,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
April 1982,
Nucleic acids research,
C T Wreesmann, and
A Fidder, and
G A van der Marel, and
J H van Boom
August 1998,
Biochemical and biophysical research communications,
