BIOMAT Database Logo BIOMAT Database Logo

Isolation of cDNA and genomic DNA clones encoding type II collagen. 1984

M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel

A cDNA library constructed from total chick embryo RNA was screened with an enriched fraction of type II collagen mRNA. Two overlapping cDNA clones were characterized and shown to encode the COOH propeptide of type II collagen. In addition, a type II collagen clone was isolated from a Charon 4A library of chick genomic fragments. Definitive identification of the clones was based on DNA sequence analysis. The 3' end of the type II collagen gene appears to be similar to that of other interstitial collagen genes. Northern hybridization data indicates that there is a marked decrease in type II collagen mRNA levels in chondrocytes treated with the dedifferentiating agent 5-bromodeoxyuridine. The major type II collagen mRNA species is 5300 bases long, similar to that of other interstitial collagen RNAs.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011061 Poly A A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Adenine Polynucleotides,Polyadenylic Acids,Poly(rA),Polynucleotides, Adenine
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli

Related Publications

M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II.
October 1995, Nucleic acids research,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I.
June 1996, Biochimica et biophysica acta,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation of cDNA and genomic clones for apolipoprotein C-II.
January 1986, Methods in enzymology,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of tomato cDNA and genomic clones encoding the ubiquitin gene ubi3.
December 1991, Plant molecular biology,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation of cDNA clones and genomic DNA clones of beta-subunit of chicken prolyl 4-hydroxylase.
January 1988, Connective tissue research,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of genomic and cDNA clones encoding mouse senescence marker protein-30 (SMP30).
July 1996, Biochimica et biophysica acta,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of cDNA clones encoding aldose reductase.
October 1989, Current eye research,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of cDNA clones encoding jacalin isolectins.
March 1993, The Journal of biological chemistry,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Analysis of cDNA and genomic clones coding for the pro alpha 1 chain of calf type II collagen.
April 1985, Nucleic acids research,
M F Young, and G Vogeli, and A M Nunez, and M P Fernandez, and M Sullivan, and M E Sobel
Isolation and characterization of cDNA clones and a genomic clone encoding rat mitochondrial adenine nucleotide translocator.
October 1993, Biochimica et biophysica acta,
Copied contents to your clipboard!