-transparent.png){kind=logo}
A totally synthetic molecule (109-145 peptide) analogous to the beta-subunit carboxyl terminus was used as an antigen in the development of antibodies by the hybridoma technique. A monoclonal antibody (702 D7) specifically recognized the free native beta-human CG (beta hCG). 702 D7 was of the immunoglobulin G1 subclass and was directed against an antigenic site localized in a 10-amino acid sequence (109-118) or less. The recognition of an epitope located in the 109-118 region could explain the specific recognition of beta hCG observed with 702 D7, in contrast to monoclonal antibodies directed against a 118-145 region with a recognition of both beta hCG and whole hCG, as observed with a second monoclonal antibody (1032) to synthetic peptide. Immunohistochemical results and preliminary data obtained from the immunoradiometric assay show that 702 D7 provides a clinical tool for the detection of free beta-subunit secretion even at low concentrations, and could allow the study of this subunit or its metabolites produced by normal and tumoral cells.
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
October 2002,
Hybridoma and hybridomics,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
February 1979,
Endocrinology,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
December 1988,
Biulleten' eksperimental'noi biologii i meditsiny,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
July 2006,
Immunology letters,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
October 1982,
Endocrinologia japonica,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
December 1983,
Nihon Sanka Fujinka Gakkai zasshi,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
December 1983,
American journal of obstetrics and gynecology,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
January 1990,
Folia biologica,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
July 2008,
The Journal of reproductive medicine,
D Bellet, and
J M Bidart, and
M Jolivet, and
A Tartar, and
J M Caillaud, and
M Ozturk, and
M C Strugo, and
F Audibert, and
H Gras-Masse, and
M Assicot
August 1986,
Journal of immunological methods,
