BIOMAT Database Logo BIOMAT Database Logo

2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences. 1984

J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg

A new approach is presented for the indirect hybridocytochemical localization of specific nucleic acid sequences in microscopic preparations. The method is based on the application of probes modified with N-acetoxy-2-acetylaminofluorene. After hybridization, the 2-acetylaminofluorene-labelled probes are recognized by antibodies directed against modified guanosine and visualized immunocytochemically. This procedure has been optimized on two model objects: mouse satellite DNA in interphase nuclei and chromosomes, and kinetoplast DNA in Crithidia fasciculata. A first application that may be of clinical importance is given by the detection of human cytomegalovirus in infected human lung fibroblasts. Other potentials of this procedure are discussed. Its advantages are: (1) the simple, rapid and reproducible labelling procedure; (2) the high stability of both label and modified probes; (3) the feasibility of labelling both double-stranded (ds) and single-stranded (ss) probes (DNA as well as RNA); (4) the rapid and sensitive detection of hybrids.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D002875 Chromosomes In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Chromosome
D003421 Crithidia A genus of parasitic protozoans found in the digestive tract of invertebrates, especially insects. Organisms of this genus have an amastigote and choanomastigote stage in their life cycle. Crithidias
D003587 Cytomegalovirus A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS. Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004276 DNA, Satellite Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION. Satellite DNA,Satellite I DNA,DNA, Satellite I,DNAs, Satellite,DNAs, Satellite I,I DNA, Satellite,I DNAs, Satellite,Satellite DNAs,Satellite I DNAs
D004277 DNA, Single-Stranded A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000099 Acetoxyacetylaminofluorene An alkylating agent that forms DNA ADDUCTS at the C-8 position in GUANINE, resulting in single strand breaks. It has demonstrated carcinogenic action. Acetoxyacetamidofluorene,Acetoxyfluorenylacetamide,N-Acetoxy-2-acetylaminofluorene,N-Acetoxy-N-acetyl-2-aminofluorene,N Acetoxy 2 acetylaminofluorene,N Acetoxy N acetyl 2 aminofluorene

Related Publications

J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Hybridocytochemistry with 2-acetylaminofluorene-modified probes.
March 1984, Cell biology international reports,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Rapid and specific detection of Salmonella infections using chemically modified nucleic acid probes.
April 2019, Analytica chimica acta,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Monoclonal antibody detection of 2-acetyl-aminofluorene-modified DNA probes for the specific detection of nucleic acids in hybridization procedures.
January 1985, Annales de l'Institut Pasteur. Immunologie,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection.
April 2021, RSC chemical biology,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Fluorescent hybridization probes for nucleic acid detection.
April 2012, Analytical and bioanalytical chemistry,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Specific nucleic acid detection using photophysical properties of quantum dot probes.
February 2010, Analytical chemistry,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Modular probes for enriching and detecting complex nucleic acid sequences.
December 2017, Nature chemistry,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Gold nanoparticle probes for the detection of nucleic acid targets.
January 2006, Clinica chimica acta; international journal of clinical chemistry,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
The detection and quantitation of SV40 nucleic acid sequences using single-stranded SV40 DNA probes.
January 1975, Cold Spring Harbor symposia on quantitative biology,
J E Landegent, and N Jasen in de Wal, and R A Baan, and J H Hoeijmakers, and M Van der Ploeg
Detection of bovine adenovirus nucleic acid sequences in nasal specimens by biotinylated DNA probes.
June 1992, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
Copied contents to your clipboard!