BIOMAT Database Logo BIOMAT Database Logo

Quantitative evaluation of two distinct cell populations expressing HLA-DR antigens in normal human epidermis. 1984

C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet

HLA-DR antigens are expressed only by Langerhans cells (LC) and indeterminate cells (IC) in normal human epidermis. Indirect immunofluorescence and ultrastructural immunogold labelling were used to study the HLA-DR expression by means of two anti-DR monoclonal antibodies (MCA). Freshly dispersed DR-positive epidermal cells expressed different densities of DR antigens on their membrane surface. Approximately 25% of DR-positive cells were strongly labelled by anti-DR-MCA and 75% were weakly stained. After 18 h in complete culture medium before labelling no significant difference in these percentages was observed. The lymphoid-like LC-enriched cells obtained by Ficoll-Hypaque centrifugation also had two populations of DR-positive cells: strongly labelled cells (30.8%) and weakly labelled cells (69.2%). DR-positive cells may be divided into two types according to the density of DR sites on their cell membrane: (I) type DR+ shows weak surface labelling by gold particles (8.8 +/- 3.0 gold particles/micron) and has cytoplasmic Birbeck granules, identifying such cells as typical Langerhans cells; (2) type DR shows strong membrane immunogold labelling (38.9 +/- 4.6 gold particles/micron) and may or may not contain Birbeck granules. The gold particles bound to the cell membrane were used to quantify the number of HLA-DR sites expressed on each cell type: 1 X 10(5) sites on DR+ cells and 5 X 10(5) on DR cells.

UI MeSH Term Description Entries
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004817 Epidermis The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006684 HLA-DR Antigens A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS. HLA-DR,Antigens, HLA-DR,HLA DR Antigens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000078404 Epidermal Cells Cells from the outermost, non-vascular layer (EPIDERMIS) of the skin. Epidermal Cell,Epidermic Cells,Cell, Epidermal,Cell, Epidermic,Cells, Epidermic,Epidermic Cell
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Demonstration of HLA-DR antigens in normal human brain.
April 1984, Journal of neurology, neurosurgery, and psychiatry,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations.
September 1984, Journal of immunology (Baltimore, Md. : 1950),
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Langerhans cells expressing HLA-DQ, HLA-DR and T6 antigens in normal oral mucosa and lichen planus.
December 1989, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Demonstration of two distinct light chains in HLA-DR-associated antigens by two-dimensional gel electrophoresis.
March 1982, European journal of immunology,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Two distinct p53 immunohistochemical patterns in human squamous-cell skin cancer, precursors and normal epidermis.
June 1996, International journal of cancer,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Two distinct phenotypes of HLA-DR-specific cytotoxic T-cell lines.
January 1983, Immunogenetics,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Heterogeneity of HLA-DR+ cells in normal human kidney. Immunohistological and cytochemical characterisation of discrete cell populations.
July 1983, Journal of clinical pathology,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Human myeloid progenitor cells expressing HLA antigens.
April 1979, Blood,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Langerhans' cell and vitiligo: quantitative study of T6 and HLA-DR antigen-expressing cells.
January 1984, Acta dermato-venereologica,
C Dezutter-Dambuyant, and G Cordier, and D Schmitt, and M Faure, and C Laquoi, and J Thivolet
Intercellular distribution of HLA-DR and T6 antigens in the epidermis of various dermatoses.
December 1985, The British journal of dermatology,
Copied contents to your clipboard!