Biomaterial Database

A molecular hybrid of the H-2Dd and H-2Ld genes expressed in the dm1 mutant. 1984

S S Burnside, and P Hunt, and K Ozato, and D W Sears

Sequential immunoprecipitates show that H-2dm1 mutant cells express a hybrid "H-2D/L" antigen exhibiting determinants normally associated with two different gene products of the parental d haplotype-i.e., the H-2Dd and H-2Ld antigens. The hybrid H-2D/Ldm1 antigen appears to consist of a portion of the NH2-terminal extracellular half of the H-2Dd antigen "fused" to a portion of the COOH-terminal extracellular half of the H-2Ld antigen. This structure is inferred from the reactivity of dm1 antigens with cytotoxic T lymphocytes specific for H-2Ld determinants and with monoclonal antibodies specific for determinants in the structural domains of H-2Ld or H-2Dd. The H-2D/Ldm1 molecule apparently retains all of the third external domain (C2 or alpha 3) and part of the second external domain (C1 or alpha 2) of H-2Ld, but its first external domain (N or alpha 1) derives from H-2Dd. From these findings and from previous peptide mapping studies, we propose that the H-2D/Ldm1 antigen is the product of a hybrid gene that has resulted from an unequal crossover between the parental H-2Dd and H-2Ld genes, leaving the N exon and part of the C1 exon of the H-2Dd gene joined to the H-2Ld gene beginning somewhere within its C1 exon.

UI	MeSH Term	Description	Entries
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D009693	Nucleic Acid Hybridization	Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)	Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D005796	Genes	A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.	Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D006183	H-2 Antigens	The major group of transplantation antigens in the mouse.	H2 Antigens,Antigens, H-2,Antigens, H2,H 2 Antigens
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic