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Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization at low temperatures was studied. The antibody activity of antibody was not affected by irradiation at an irradiation dose of below 8 MR and low temperatures. Immobilization of peroxidase-labeled anti-rabbit IgG goat IgG, anti-peroxidase, peroxidase, and anti-alpha-fetoprotein was carried out with hydrophilic and hydrophobic monomers. The activity of the immobilized enzyme-labeled antibody membranes varied with the thickness of the membranes and increased with decreasing membrane thickness. The activity of the immobilized antibody particles was varied by particle size. Immobilized anti-alpha-fetoprotein particles and membranes can be used for the assay of alpha-fetoprotein by the antigen-antibody reaction, such as a solid-phase sandwich method with high sensitivity.
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M Kumakura, and
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April 2011,
Analytical chemistry,
M Kumakura, and
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April 2019,
Water science and technology : a journal of the International Association on Water Pollution Research,
M Kumakura, and
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January 1992,
Methods of biochemical analysis,
M Kumakura, and
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June 1990,
Agricultural and biological chemistry,
M Kumakura, and
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