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A study of the solubilization of glomerular deposition of IgG immune complexes by sera from patients with lupus nephritis is described. Renal biopsy specimens were obtained from 11 patients with lupus nephritis, five patients with IgA nephropathy and one patient with minimal change nephrotic syndrome. These renal specimens were incubated with fresh, stored or heated sera from the same patients or healthy adults and human gamma-globulins at 37 degrees C for 1 h in plastic test tubes. The sections were stained with FITC conjugated heavy chain specific anti-human IgG or C3 antisera and then examined with a fluorescent microscope. The sections were also stained with FITC conjugated human gamma-globulins and rhodamine conjugated anti-human IgG, IgM or IgA antisera and then examined by double exposure under a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulins significantly solubilize glomerular immune deposits in patients with lupus nephritis in vitro. It was indicated that the solubilization of IgG glomerular deposits from patients with lupus nephritis does not depend on complement. It is postulated that solubilization of immune deposits in glomeruli requires the excess amounts of antigenic substances in patients with lupus nephritis.
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
September 1992,
Pharmacological research,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
December 1984,
Clinical and experimental immunology,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
September 1976,
Kidney international,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
May 1999,
American journal of kidney diseases : the official journal of the National Kidney Foundation,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
August 1983,
The Tohoku journal of experimental medicine,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
January 1981,
Arthritis and rheumatism,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
July 1993,
The American journal of pathology,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
January 1991,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
December 1981,
Clinical and experimental immunology,
Y Tomino, and
H Sakai, and
M Takaya, and
M Miura, and
T Suga, and
M Endoh, and
Y Nomoto
August 1989,
Nihon Jinzo Gakkai shi,
