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We have prepared and cloned cDNAs derived from poly(A)+ RNA from a human pulmonary carcinoid tumor rich in immunoreactivity to gastrin-releasing peptide, a peptide closely related in structure to amphibian bombesin. Mixtures of synthetic oligodeoxyribonucleotides corresponding to amphibian bombesin were used as hybridization probes to screen a cDNA library prepared from the tumor RNA. Sequencing of the recombinant plasmids shows that human gastrin-releasing peptide (hGRP) mRNA encodes a precursor of 148 amino acids containing a typical signal sequence, hGRP consisting of 27 or 28 amino acids, and a carboxyl-terminal extension peptide. hGRP is flanked at its carboxyl terminus by two basic amino acids, following a glycine used for amidation of the carboxyl-terminal methionine. RNA blot analyses of tumor RNA show a major mRNA of 900 bases and a minor mRNA of 850 bases. Blot hybridization analyses using human genomic DNA are consistent with a single hGRP-encoding gene. The presence of two mRNAs encoding the hGRP precursor protein in the face of a single hGRP gene raises the possibility of alternative processing of the single RNA transcript.
E R Spindel, and
W W Chin, and
J Price, and
L H Rees, and
G M Besser, and
J F Habener
December 1990,
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E R Spindel, and
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L H Rees, and
G M Besser, and
J F Habener
January 1988,
Annals of the New York Academy of Sciences,
E R Spindel, and
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G M Besser, and
J F Habener
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E R Spindel, and
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E R Spindel, and
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E R Spindel, and
W W Chin, and
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J F Habener
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E R Spindel, and
W W Chin, and
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L H Rees, and
G M Besser, and
J F Habener
January 1986,
Gene,
E R Spindel, and
W W Chin, and
J Price, and
L H Rees, and
G M Besser, and
J F Habener
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DNA and cell biology,
E R Spindel, and
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J Price, and
L H Rees, and
G M Besser, and
J F Habener
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Biochemical and biophysical research communications,
E R Spindel, and
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J Price, and
L H Rees, and
G M Besser, and
J F Habener
January 1999,
DNA sequence : the journal of DNA sequencing and mapping,
