-transparent.png){kind=logo}
Spleen cells of BALB/c mice immunized with human chorionic gonadotropin (HCG), a glycopeptide hormone composed of two nonidentical alpha and beta subunits, were fused with NS-1 mouse myeloma cells. Two hybrid cell lines, cone PC2 and clone PD3 secreting monoclonal antibodies (McAb) to HCG were isolated. With the aid of a double antibody radioimmunoassay it was established that PC2-McAb recognizes an immunodeterminant associated with beta-HCG subunit, whereas, PD3-McAb recognizes an epitope present on the alpha-HCG subunit. Both monoclonal antibodies showed an affinity constant of approximately 5 X 10(10) L/M. Because of these immunological characteristics, only PC2-McAb specifically reacts with HCG and shows cross-reactivities of less than 0.1% to other related human glycopeptide hormones. On the other hand, using a hemagglutination test based on antibody induced agglutination of sheep red blood cells coated with HCG, it was shown that only PD3-McAb is capable of inducing a positive reaction, although both monoclonal antibodies have a similar binding capacity to the coated cells. The potential of these two new monoclonal reagents for the qualitative detection and quantitative determination of HCG in human biological fluids is discussed.
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
January 1998,
Asian Pacific journal of allergy and immunology,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
December 1988,
Biulleten' eksperimental'noi biologii i meditsiny,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
September 1984,
Archives of pathology & laboratory medicine,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
January 1986,
Diagnostic immunology,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
May 1990,
The Journal of endocrinology,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
May 1990,
Wiener klinische Wochenschrift,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
December 1983,
Nihon Sanka Fujinka Gakkai zasshi,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
July 1972,
Biochemical and biophysical research communications,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
January 1978,
Current topics in experimental endocrinology,
L Wang, and
N Rahamim, and
N Harpaz, and
C S Hexter, and
M Inbar
February 1987,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
