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We screened several synthetic polyprenoids about their capacity of cell differentiation using mouse myeloid leukemic M1 cells, and found that 3, 7, 11, 15, 19,-pentamethyleicosa-2, 6, 10, 14, 18-pentaenol (Geranyl Farnesol) could induce the differentiation of M1 cells into macrophage-like cells. The optimal concentration of this compound for its induction capacity was approximately 2 X 10(-5) M. The induced differentiation related markers were morphological change in M1 cells, Fc receptors for IgG (Fc gamma), and non-specific esterase activity. However, phagocytic activity was poorly induced. This suggests that most of Geranyl Farnesol treated cells may be retained functionally incomplete macrophages. The presence of cycloheximide at 1 X 10(-7)M or the incubation of M1 cells at 4 degrees C completely blocked the induction of Fc gamma, suggesting the induction of Fc gamma required protein synthesis. The treatment of M1 cells with Geranyl Farnesol resulted in suppression of DNA and RNA synthesis. Further analysis of RNA metabolism suggested that the suppression of RNA synthesis was mainly due to the decrease of rRNA. On the other hand the synthesis and turnover of polyA-containing RNA (mRNA) rather increased.
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
January 1983,
Leukemia research,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
May 1986,
Experimental cell research,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
October 1986,
FEBS letters,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
September 1984,
The Journal of biological chemistry,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
August 1996,
Experimental hematology,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
February 1983,
Cell differentiation,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
August 1979,
Biochemical and biophysical research communications,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
August 1989,
The Journal of biological chemistry,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
August 1980,
Cancer research,
H Ishikura, and
T Mochizuki, and
Y Izumi, and
T Usui, and
H Sawada, and
H Uchino
April 1992,
Cellular immunology,
