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A 5-step fractionation procedure was used to isolate a prostatic growth factor from a tissue homogenate of human benign prostatic hyperplasia. The procedure made use of the property of prostatic growth factor to occur predominately as a large molecule (greater than 67,000) in low ionic strength solution (Tris with 0.05 M NaCl) but as a smaller molecule (less than 33,000) in high salt buffer (Tris with 1.55 M NaCl). The fractionation scheme consisted of ammonium sulfate precipitation, gel filtration (low ionic strength) and DEAE chromatography. The growth factor activity was eluted from DEAE with high ionic strength buffer followed by 2 gel filtration steps in high ionic strength buffer. The fractionation scheme produced greater than a 200-fold increase in prostatic growth factor activity and 10 per cent recovery. Additional separation steps will be required to purify prostatic growth factor to homogeneity.
M T Story, and
S C Jacobs, and
R K Lawson
November 1985,
Biochemical and biophysical research communications,
M T Story, and
S C Jacobs, and
R K Lawson
January 1992,
Urological research,
M T Story, and
S C Jacobs, and
R K Lawson
January 1988,
The Prostate,
M T Story, and
S C Jacobs, and
R K Lawson
January 1986,
European urology,
M T Story, and
S C Jacobs, and
R K Lawson
January 1987,
Cancer research,
M T Story, and
S C Jacobs, and
R K Lawson
February 1989,
Archives of biochemistry and biophysics,
M T Story, and
S C Jacobs, and
R K Lawson
August 1979,
Proceedings of the National Academy of Sciences of the United States of America,
M T Story, and
S C Jacobs, and
R K Lawson
January 1996,
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao,
M T Story, and
S C Jacobs, and
R K Lawson
January 1987,
Archivum immunologiae et therapiae experimentalis,
M T Story, and
S C Jacobs, and
R K Lawson
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
