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RNA-dependent DNA-polymerase activity was found in the 165 000 g supernatant and pellet of the postmitochondrial rat liver fraction. Further fractionation of the 165 000 g pellet in the linear sucrose gradient (20-50%) showed that RNA-dependent DNA-polymerase activity was distributed between fractions with densities 1.18-1.19 g/ml and 1.09-1.1 g/ml. In the fractions with 1.18-1.19 g/ml density the enzymic activity could be detected only after Triton X-100 treatment and disappeared after the incubation with pancreatic ribonuclease A. Triton X-100 treatment of the 165 000 g supernatant and the fractions with density 1.09-1.1 g/ml did not increase further the enzymic activity. Electron microscopy revealed in the 1.18 g/ml fraction virus-like particles resembling retroviruses of A and C type. In the light peak "non-mature" virus-like particles were found. The 165 000 g supernatant devoid of virus-like particles contained free RNA-dependent DNA-polymerase activity. The virus-like particles of both types seem to be endogenous rat retroviruses serving as a source of the particular and free reverse transcriptase in the rat liver.
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
January 1984,
Molekuliarnaia biologiia,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
May 1971,
Nature,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
April 1972,
FEBS letters,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
November 1973,
Proceedings of the National Academy of Sciences of the United States of America,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
November 1967,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
July 1974,
Doklady Akademii nauk SSSR,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
August 1971,
Nature: New biology,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
January 1969,
European journal of biochemistry,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
May 1972,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G B Pyrinova, and
N P Korokhov, and
E V Kiseleva, and
V P Tomsons, and
N B Khristoliubova
January 1974,
Methods in enzymology,
