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Attempts have been made to make monoclonal antibodies which would react preferentially with either the concanavalin A (con A) binding or non-binding from of human alphafetoprotein (AFP). Mice were immunised with con A non-binding AFP in a variety of schedules, and their spleens used in four separate fusions. Despite identification of 467 hybridomas secreting anti-AFP antibodies, no monoclonals could be found with consistent or reproducible specificity for either con A binding or non-binding AFP. Ten of the hybridomas were grown as ascitic tumours. The resulting ascitic fluids defined 4 discrete epitopes on the AFP molecule. There was no apparent difference in the binding of a particular monoclonal antibody to its target epitope in the con A binding and non-binding forms of AFP. This finding is discussed in the light of current knowledge of the molecular structure of the carbohydrate chains of con A binding and non-binding forms of AFP.
D J Brock, and
L Barron, and
V Van Heyningen
January 1979,
Journal of supramolecular structure,
D J Brock, and
L Barron, and
V Van Heyningen
February 1988,
Science (New York, N.Y.),
D J Brock, and
L Barron, and
V Van Heyningen
August 2002,
Hybridoma and hybridomics,
D J Brock, and
L Barron, and
V Van Heyningen
November 1983,
Clinica chimica acta; international journal of clinical chemistry,
D J Brock, and
L Barron, and
V Van Heyningen
June 1979,
FEBS letters,
D J Brock, and
L Barron, and
V Van Heyningen
January 1981,
Intervirology,
D J Brock, and
L Barron, and
V Van Heyningen
September 1986,
Scandinavian journal of immunology,
D J Brock, and
L Barron, and
V Van Heyningen
December 1983,
Molecular immunology,
D J Brock, and
L Barron, and
V Van Heyningen
December 2010,
Glycobiology,
D J Brock, and
L Barron, and
V Van Heyningen
June 1989,
Hybridoma,
