Biomaterial Database

Comparison of dengue viruses and some other flaviviruses by cDNA-RNA hybridization analysis and detection of a close relationship between dengue virus serotype 2 and Edge Hill virus. 1984

J Blok, and E A Henchal, and B M Gorman

Variable amounts of cDNA were synthesized in vitro from RNA extracted from several flaviviruses, including the four prototype dengue (DEN) virus serotypes. The synthesis was carried out using an oligo(dT) primer, suggesting the presence of a short poly(A) region at or near the 3' end of some flavivirus genomes. The DEN-1 and DEN-2 prototype strains produced the largest amount of cDNA and were therefore used to investigate further the relatedness of flavivirus genomes by cDNA-RNA hybridization. The flaviviruses studied are related to each other to some extent since the hybrids formed exhibited about 30% S1 nuclease resistance, but a closer relationship was detected between dengue viruses of serotype 1 and 4 and between dengue virus serotype 2 and Edge Hill virus. A monoclonal antibody to the envelope protein (V3) of dengue viruses reacted with Edge Hill virus, confirming the genetic relationship between the viruses.

UI	MeSH Term	Description	Entries
D009693	Nucleic Acid Hybridization	Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)	Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D003716	Dengue Virus	A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known.	Breakbone Fever Virus,Breakbone Fever Viruses,Dengue Viruses,Fever Virus, Breakbone,Fever Viruses, Breakbone,Virus, Breakbone Fever,Virus, Dengue,Viruses, Breakbone Fever,Viruses, Dengue
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005416	Flavivirus	A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS.	Arbovirus, Group B,Arboviruses, Group B,Flaviviruses,Group B Arbovirus,Group B Arboviruses
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA